Network reconstruction and systems analysis of plant cell wall deconstruction by

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Biotechnol Biofuels


Biofuels; CLR-2; Mannan; Network reconstruction; Neurospora crassa; Plant cell wall degradation network; Systems biology; Transcriptional regulatory networks


BACKGROUND: Plant biomass degradation by fungal-derived enzymes is rapidly expanding in economic importance as a clean and efficient source for biofuels. The ability to rationally engineer filamentous fungi would facilitate biotechnological applications for degradation of plant cell wall polysaccharides. However, incomplete knowledge of biomolecular networks responsible for plant cell wall deconstruction impedes experimental efforts in this direction.

RESULTS: To expand this knowledge base, a detailed network of reactions important for deconstruction of plant cell wall polysaccharides into simple sugars was constructed for the filamentous fungus

CONCLUSIONS: Here we built a network that serves as a scaffold for integration of diverse experimental datasets. This approach led to the elucidation of regulatory design principles for plant cell wall deconstruction by filamentous fungi and a novel function for the transcription factor CLR-2. This expanding network will aid in efforts to rationally engineer industrially relevant hyper-production strains.


Institute for Systems Biology