Single-Cell Glia and Neuron Gene Expression in the Central Amygdala in Opioid Withdrawal Suggests Inflammation With Correlated Gut Dysbiosis.

Publication Title

Front Neurosci

Authors

Sean J O'Sullivan
Evangelia Malahias
James Park, Institute for Systems Biology, Seattle, WA, United States
Ankita Srivastava
Beverly A S Reyes
Jon Gorky
Rajanikanth Vadigepalli
Elisabeth J Van Bockstaele
James S Schwaber

Document Type

Article

Publication Date

1-1-2019

Abstract

Drug-seeking in opioid dependence is due in part to the severe negative emotion associated with the withdrawal syndrome. It is well-established that negative emotional states emerge from activity in the amygdala. More recently, gut microflora have been shown to contribute substantially to such emotions. We measured gene expression in single glia and neurons gathered from the amygdala using laser capture microdissection and simultaneously measured gut microflora in morphine-dependent and withdrawn rats to investigate drivers of negative emotion in opioid withdrawal. We found that neuroinflammatory genes, notably

Clinical Institute

Neurosciences (Brain & Spine)

Specialty

Institute for Systems Biology

Specialty

Neurosciences

Recommended Citation

O'Sullivan, Sean J; Malahias, Evangelia; Park, James; Srivastava, Ankita; Reyes, Beverly A S; Gorky, Jon; Vadigepalli, Rajanikanth; Van Bockstaele, Elisabeth J; and Schwaber, James S, "Single-Cell Glia and Neuron Gene Expression in the Central Amygdala in Opioid Withdrawal Suggests Inflammation With Correlated Gut Dysbiosis." (2019). Articles, Abstracts, and Reports. 1937.
https://digitalcommons.providence.org/publications/1937