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Methods in molecular biology (Clifton, N.J.)


Algorithms; Alternative Splicing; Animals; Computational Biology; Genome-Wide Association Study; Humans; Mice; Models, Molecular; Molecular Sequence Annotation; Protein Conformation; Protein Folding; Protein Isoforms; Proteins; Quantitative Structure-Activity Relationship; Software; Web Browser; Workflow


Tens of thousands of splice isoforms of proteins have been catalogued as predicted sequences from transcripts in humans and other species. Relatively few have been characterized biochemically or structurally. With the extensive development of protein bioinformatics, the characterization and modeling of isoform features, isoform functions, and isoform-level networks have advanced notably. Here we present applications of the I-TASSER family of algorithms for folding and functional predictions and the IsoFunc, MIsoMine, and Hisonet data resources for isoform-level analyses of network and pathway-based functional predictions and protein-protein interactions. Hopefully, predictions and insights from protein bioinformatics will stimulate many experimental validation studies.


Institute for Systems Biology