Title
Platelet derived growth factor receptor β (PDGFRβ) is a host receptor for the human malaria parasite adhesin TRAP.
Document Type
Article
Publication Date
5-31-2021
Publication Title
Sci Rep
Keywords
washington; seattle
Abstract
Following their inoculation by the bite of an infected Anopheles mosquito, the malaria parasite sporozoite forms travel from the bite site in the skin into the bloodstream, which transports them to the liver. The thrombospondin-related anonymous protein (TRAP) is a type 1 transmembrane protein that is released from secretory organelles and relocalized on the sporozoite plasma membrane. TRAP is required for sporozoite motility and host infection, and its extracellular portion contains adhesive domains that are predicted to engage host receptors. Here, we identified the human platelet-derived growth factor receptor β (hPDGFRβ) as one such protein receptor. Deletion constructs showed that the von Willebrand factor type A and thrombospondin repeat domains of TRAP are both required for optimal binding to hPDGFRβ-expressing cells. We also demonstrate that this interaction is conserved in the human-infective parasite Plasmodium vivax, but not the rodent-infective parasite Plasmodium yoelii. We observed expression of hPDGFRβ mainly in cells associated with the vasculature suggesting that TRAP:hPDGFRβ interaction may play a role in the recognition of blood vessels by invading sporozoites.
Clinical Institute
Women & Children
Department
Institute for Systems Biology
Department
Allergy & Immunology
Department
Infectious Diseases
Recommended Citation
Steel, Ryan W J; Vigdorovich, Vladimir; Dambrauskas, Nicholas; Wilder, Brandon K; Arredondo, Silvia A; Goswami, Debashree; Kumar, Sudhir; Carbonetti, Sara; Swearingen, Kristian E; Nguyen, Thao; Betz, Will; Camargo, Nelly; Fisher, Bridget S; Soden, Jo; Thomas, Helen; Freeth, Jim; Moritz, Robert L; Noah Sather, D; and Kappe, Stefan H I, "Platelet derived growth factor receptor β (PDGFRβ) is a host receptor for the human malaria parasite adhesin TRAP." (2021). Articles, Abstracts, and Reports. 4895.
https://digitalcommons.providence.org/publications/4895