Title

Protein Catalyzed Capture (PCC) Agents for Antigen Targeting.

Authors

Matthew N Idso, Institute for Systems Biology, Seattle, WA, USA.
Bert T Lai
Heather D Agnew
James R Heath, Institute for Systems Biology, Seattle, USAFollow

Document Type

Article

Publication Date

1-1-2022

Publication Title

Methods in molecular biology (Clifton, N.J.)

Keywords

washington; seattle; isb

Abstract

The protein catalyzed capture agent (PCC) method is a powerful combinatorial screening strategy for discovering synthetic macrocyclic peptide ligands, called PCCs, to designated protein epitopes. The foundational concept of the PCC method is the use of in situ click chemistry to survey large combinatorial libraries of peptides for ligands to designated biological targets. State-of-the-art PCC screens integrate synthetic libraries of constrained macrocyclic peptides with epitope-specific targeting strategies to identify high-affinity (<100 >nM) binders de novo. Automated instrumentation can accelerate PCC discovery to a rapid 2-week timeframe. Here, we describe methods to perform combinatorial screens that yield epitope-targeted PCCs.

Department

Institute for Systems Biology

Recommended Citation

Idso, Matthew N; Lai, Bert T; Agnew, Heather D; and Heath, James R, "Protein Catalyzed Capture (PCC) Agents for Antigen Targeting." (2022). Articles, Abstracts, and Reports. 5342.
https://digitalcommons.providence.org/publications/5342