Tumor resident memory CD8 T cells and concomitant tumor immunity develop independently of CD4 help.
oregon; chiles; portland
Tissue resident memory (Trm) CD8 T cells infiltrating tumors represent an enriched population of tumor antigen-specific T cells, and their presence is associated with improved outcomes in patients. Using genetically engineered mouse pancreatic tumor models we demonstrate that tumor implantation generates a Trm niche that is dependent on direct antigen presentation by cancer cells. However, we observe that initial CCR7-mediated localization of CD8 T cells to tumor draining lymph nodes is required to subsequently generate CD103+ CD8 T cells in tumors. We observe that the formation of CD103+ CD8 T cells in tumors is dependent on CD40L but independent of CD4 T cells, and using mixed chimeras we show that CD8 T cells can provide their own CD40L to permit CD103+ CD8 T cell differentiation. Finally, we show that CD40L is required to provide systemic protection against secondary tumors. These data suggest that CD103+ CD8 T cell formation in tumors can occur independent of the two-factor authentication provided by CD4 T cells and highlight CD103+ CD8 T cells as a distinct differentiation decision from CD4-dependent central memory.
Earle A. Chiles Research Institute
Medler, Terry R; Kramer, Gwen; Bambina, Shelly; Gunderson, Andrew J; Alice, Alejandro; Blair, Tiffany; Zebertavage, Lauren; Duhen, Thomas; Duhen, Rebekka; Young, Kristina; Crittenden, Marka R; and Gough, Michael J, "Tumor resident memory CD8 T cells and concomitant tumor immunity develop independently of CD4 help." (2023). Articles, Abstracts, and Reports. 7235.