Event Title
Changes to Gut Microbiota in Nurses after a 12-Hour Shift Versus after a Full Day off Work
Location
Central Division
Start Date
26-10-2023 9:05 AM
End Date
26-10-2023 9:15 AM
Description
Abstract:
Title: Changes to Gut Microbiota in Nurses after a 12-Hour Shift Versus after a Full Day off Work
Authors: Tommy Elliott, Elena Carter, Sam Downey, Daniel Shay, and Teresa Rangel
Objectives: After attending this session, learners will:
1. Describe one health function of the human gut microbiome
2. Link stress to changes in the human gut microbiome
3. Articulate at least one health implication of shifted gut microbiome composition among nurses
Background: Nursing care in the hospital setting can be demanding, creating occupational stress. Gastrointestinal issues frequently accompany symptoms of stress, presenting significant health complications long-term. Chronic stress is also linked to obesity, a condition prevalent among nurses. The gut microbiome is composed of several bacterial genera which may mediate health states and the composition of the gut microbiome may shift in as little as 24 hours due to changes such as acute and chronic stress. Certain genera in the gut microbiome may be advantageous, such as lactobacillus which promotes food digestion, and parabacteroides which produce the inhibitory neurotransmitter, GABA, which can reduce depressive symptoms. Other phyla and genera, including higher firmicutes-bacteroidetes (F/B) ratios and lactobacillus, have been implicated in adverse health states such as obesity and an imbalanced immune system. More research is imperative to determine mechanisms linking occupational stress to gut microbiome changes in acute care nurses.
Purpose: To determine how the gut microbiome alters among nurses after a 12-hour hospital shift compared to a full day off work.
Methods: This secondary analysis analyzed a sub-set of fecal samples provided by 30 acute care, frontline nurses between the years 2020-2021. Nurses in the parent study provided two samples within a 7-day period: one within 24 hours of finishing a 12-hour shift and the other within 24 hours of finishing a full day off work. DNA from 6 participants was prepared and underwent whole-genome sequencing. Resulting metadata was analyzed with Pavian. The F/B ratio was calculated for both conditions and defined as elevated if above 3.
Results: The 6 nurse participants with data analyzed indicated significantly higher concentrations of several bacterial genera for “on” versus “off” conditions: parabacteroides, lactobacillus, and staphylococcus. The firmicutes-bacteroidetes ratios (F/B) ranged from 0.469 to 7, with a mean of 3.0538 for "on" samples and 3.2380 for “off” samples, although differences were not found to be statistically significant (p>0.05).
Conclusion: In our secondary analysis, we present that significant differences in gut microbiome composition are present when nurses are on versus off work. Next steps include sequencing all available samples and performing multivariate analysis. The goal is to determine diversity metric differences between groups of nurses with consideration of socio-demographic and occupational characteristics to provide insight into health implications of work-related stress.
Implications for Practice: Nurses in this sub-analysis had generally elevated F/B ratios despite the on or off work condition which may suggest that nurses are at a higher risk of obesity and have perturbed gut microbiomes. Three genera, staphylococcus, parabacteroides, and lactobacillus, were found to have proliferated while working likely due to a decrease in species diversity, as a result of stress, allowing niche colonization. Increased lactobacillus compositions in nurses while working may contribute to obesity due to digestion facilitation. Increased staphylococcus compositions may indicate an increased risk of infectious illness among nurses, as this genus can invade and infect human intestinal cells.
Recommended Citation
Elliott, Thomas; Carter, Elena; Downey, Sam; Shay, Daniel; and Rangel, Teresa, "Changes to Gut Microbiota in Nurses after a 12-Hour Shift Versus after a Full Day off Work" (2023). Central Division Nurse Clinical Inquiry Conference. 13.
https://digitalcommons.providence.org/central_nurs_conf/2023/agenda/13
Changes to Gut Microbiota in Nurses after a 12-Hour Shift Versus after a Full Day off Work
Central Division
Abstract:
Title: Changes to Gut Microbiota in Nurses after a 12-Hour Shift Versus after a Full Day off Work
Authors: Tommy Elliott, Elena Carter, Sam Downey, Daniel Shay, and Teresa Rangel
Objectives: After attending this session, learners will:
1. Describe one health function of the human gut microbiome
2. Link stress to changes in the human gut microbiome
3. Articulate at least one health implication of shifted gut microbiome composition among nurses
Background: Nursing care in the hospital setting can be demanding, creating occupational stress. Gastrointestinal issues frequently accompany symptoms of stress, presenting significant health complications long-term. Chronic stress is also linked to obesity, a condition prevalent among nurses. The gut microbiome is composed of several bacterial genera which may mediate health states and the composition of the gut microbiome may shift in as little as 24 hours due to changes such as acute and chronic stress. Certain genera in the gut microbiome may be advantageous, such as lactobacillus which promotes food digestion, and parabacteroides which produce the inhibitory neurotransmitter, GABA, which can reduce depressive symptoms. Other phyla and genera, including higher firmicutes-bacteroidetes (F/B) ratios and lactobacillus, have been implicated in adverse health states such as obesity and an imbalanced immune system. More research is imperative to determine mechanisms linking occupational stress to gut microbiome changes in acute care nurses.
Purpose: To determine how the gut microbiome alters among nurses after a 12-hour hospital shift compared to a full day off work.
Methods: This secondary analysis analyzed a sub-set of fecal samples provided by 30 acute care, frontline nurses between the years 2020-2021. Nurses in the parent study provided two samples within a 7-day period: one within 24 hours of finishing a 12-hour shift and the other within 24 hours of finishing a full day off work. DNA from 6 participants was prepared and underwent whole-genome sequencing. Resulting metadata was analyzed with Pavian. The F/B ratio was calculated for both conditions and defined as elevated if above 3.
Results: The 6 nurse participants with data analyzed indicated significantly higher concentrations of several bacterial genera for “on” versus “off” conditions: parabacteroides, lactobacillus, and staphylococcus. The firmicutes-bacteroidetes ratios (F/B) ranged from 0.469 to 7, with a mean of 3.0538 for "on" samples and 3.2380 for “off” samples, although differences were not found to be statistically significant (p>0.05).
Conclusion: In our secondary analysis, we present that significant differences in gut microbiome composition are present when nurses are on versus off work. Next steps include sequencing all available samples and performing multivariate analysis. The goal is to determine diversity metric differences between groups of nurses with consideration of socio-demographic and occupational characteristics to provide insight into health implications of work-related stress.
Implications for Practice: Nurses in this sub-analysis had generally elevated F/B ratios despite the on or off work condition which may suggest that nurses are at a higher risk of obesity and have perturbed gut microbiomes. Three genera, staphylococcus, parabacteroides, and lactobacillus, were found to have proliferated while working likely due to a decrease in species diversity, as a result of stress, allowing niche colonization. Increased lactobacillus compositions in nurses while working may contribute to obesity due to digestion facilitation. Increased staphylococcus compositions may indicate an increased risk of infectious illness among nurses, as this genus can invade and infect human intestinal cells.
Comments
References:
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