An efficient solution for resolving iTRAQ and TMT channel crosstalk.
Publication Title
Journal of mass spectrometry : JMS
Document Type
Article
Publication Date
3-18-2019
Abstract
Isobaric tagging reagents such as iTRAQ and TMT typically have isotopic impurities that cause significant crosstalk between channels. Here we present an efficient solution to compensate for channel crosstalk using linear algebra and find that it is between 20x and 120x faster than previous methods. We also find that the effects of channel crosstalk are as important to manage as the effects of ratio compression due to precursor impurities, and we have released an open-source tool to perform both types of calculations.
Specialty/Research Institute
Institute for Systems Biology
COinS
