Cellular thermal shift analysis for interrogation of CRISPR-assisted proteomic changes.

Publication Title

BioTechniques

Authors

Nam-Gu Her
Ivan Babic, John Wayne Cancer Institute & Pacific Neuroscience Institute, Providence Saint John's Health Center, Santa Monica, CA 90404, USA
Venkata Yenugonda, John Wayne Cancer Institute and Pacific Neuroscience Institute at Providence Saint John's Health Center, Santa Monica, CA, USA.Follow
Santosh Kesari, Department of Translational Neuro-Oncology and Neurotherapeutics, John Wayne Cancer Institute at Providence Saint John's Health CenterFollow
Elmar Nurmemmedov, John Wayne Cancer Institute & Pacific Neuroscience Institute at Providence Saint John's Health Center, Santa Monica, CA 90404, USA.Follow

Document Type

Article

Publication Date

2-10-2020

Keywords

CETSA; CRISPR; cellular thermal shift; protein complex

Abstract

CRISPR-Cas9 has proven to be a versatile tool for the discovery of essential genetic elements involved in various disease states. CRISPR-assisted dense mutagenesis focused on therapeutically challenging protein complexes allows us to systematically perturb protein-coding sequences

Area of Special Interest

Cancer