Cellular thermal shift analysis for interrogation of CRISPR-assisted proteomic changes.
Publication Title
BioTechniques
Authors
Nam-Gu Her
Ivan Babic, John Wayne Cancer Institute & Pacific Neuroscience Institute, Providence Saint John's Health Center, Santa Monica, CA 90404, USA
Venkata Yenugonda, John Wayne Cancer Institute and Pacific Neuroscience Institute at Providence Saint John's Health Center, Santa Monica, CA, USA.Follow
Santosh Kesari, Department of Translational Neuro-Oncology and Neurotherapeutics, John Wayne Cancer Institute at Providence Saint John's Health CenterFollow
Elmar Nurmemmedov, John Wayne Cancer Institute & Pacific Neuroscience Institute at Providence Saint John's Health Center, Santa Monica, CA 90404, USA.Follow
Publication Date
2-10-2020
Keywords
CETSA; CRISPR; cellular thermal shift; protein complex
Abstract
CRISPR-Cas9 has proven to be a versatile tool for the discovery of essential genetic elements involved in various disease states. CRISPR-assisted dense mutagenesis focused on therapeutically challenging protein complexes allows us to systematically perturb protein-coding sequences
Area of Special Interest
Cancer
