Multiomic Immunophenotyping of COVID-19 Patients Reveals Early Infection Trajectories.
Publication Title
bioRxiv
Authors
Yapeng Su, Institute for Systems Biology
Daniel Chen, Institute for Systems Biology
Christopher Lausted, Institute for Systems Biology
Dan Yuan, Institute for Systems BiologyFollow
Jongchan Choi, Institute for Systems Biology
Cheng Dai, Institute for Systems Biology
Valentin Voillet
Kelsey Scherler, Institute for Systems Biology
Pamela Troisch, Institute for Systems Biology
Venkata R Duvvuri, Institute for Systems BiologyFollow
Priyanka Baloni, Institute for Systems Biology
Guangrong Qin, Institute for Systems Biology
Brett Smith, Institute for Systems BiologyFollow
Sergey Kornilov, Institute for Systems Biology
Clifford Rostomily, Institute for Systems BiologyFollow
Alex Xu, Institute for Systems Biology
Jing Li
Shen Dong
Alissa Rothchild
Jing Zhou
Kim Murray, Institute for Systems Biology
Rick Edmark, Institute for Systems Biology
Sunga Hong, Institute for Systems Biology
Lesley Jones, Institute for Systems Biology
Yong Zhou, Institute for Systems Biology
Ryan Roper, Institute for Systems BiologyFollow
Sean Mackay
D Shane O'Mahony, Department of Pulmonary and Critical Care Medicine, Swedish Medical Center, Seattle, WashingtonFollow
Christopher Dale, Swedish Health ServicesFollow
Julie A Wallick, Swedish Medical Center - Swedish Center for Research and InnovationFollow
Heather A Algren, Swedish Medical Center - Swedish Center for Research and InnovationFollow
Zager A Michael, Swedish Medical Center - Swedish Center for Research and Innovation
Andrew T Magis, Institute for Systems Biology, Seattle, Washington.Follow
Wei Wei, Institute for Systems BiologyFollow
Nathan D Price, Institute for Systems Biology, 401 Terry Avenue N, Seattle, WA, 98109, USA.Follow
Sui Huang, Institute for Systems Biology, Seattle, Washington, USA.Follow
Naeha Subramanian, Institute for Systems BiologyFollow
Kai Wang, Institute for Systems Biology
Jennifer Hadlock, Institute for Systems Biology
Leroy Hood, Institute for Systems Biology, Seattle, Washington, United States of America.Follow
Alan Aderem
Jeffrey A Bluestone
Lewis L Lanier
Phil Greenberg
Raphael Gottardo
Mark M Davis
Jason D Goldman, Division of Infectious Diseases Swedish Medical Center Seattle WA.Follow
James R Heath, Institute for Systems Biology
ISB-Swedish COVID19 Biobanking Unit
Publication Date
7-31-2020
Abstract
Host immune responses play central roles in controlling SARS-CoV2 infection, yet remain incompletely characterized and understood. Here, we present a comprehensive immune response map spanning 454 proteins and 847 metabolites in plasma integrated with single-cell multi-omic assays of PBMCs in which whole transcriptome, 192 surface proteins, and T and B cell receptor sequence were co-analyzed within the context of clinical measures from 50 COVID19 patient samples. Our study reveals novel cellular subpopulations, such as proliferative exhausted CD8 + and CD4 + T cells, and cytotoxic CD4 + T cells, that may be features of severe COVID-19 infection. We condensed over 1 million immune features into a single immune response axis that independently aligns with many clinical features and is also strongly associated with disease severity. Our study represents an important resource towards understanding the heterogeneous immune responses of COVID-19 patients and may provide key information for informing therapeutic development.
Specialty/Research Institute
Institute for Systems Biology
Specialty/Research Institute
Infectious Diseases
Specialty/Research Institute
Critical Care Medicine
