Engineered SH2 Domains for Targeted Phosphoproteomics.

Publication Title

ACS Chem Biol

Document Type

Article

Publication Date

6-17-2022

Keywords

washington; isb; Humans; Mass Spectrometry; Phosphotyrosine; Protein Binding; Proteome; src Homology Domains

Abstract

A comprehensive analysis of the phosphoproteome is essential for understanding molecular mechanisms of human diseases. However, current tools used to enrich phosphotyrosine (pTyr) are limited in their applicability and scope. Here, we engineered new superbinder Src-Homology 2 (SH2) domains that enrich diverse sets of pTyr-peptides. We used phage display to select a Fes-SH2 domain variant (superFes; sFes

Specialty/Research Institute

Institute for Systems Biology

Link to Full Text

Providence Full Text

Share

COinS