Engineered SH2 Domains for Targeted Phosphoproteomics.

Publication Title

ACS Chem Biol

Authors

Gregory D Martyn
Gianluca Veggiani
Ulrike Kusebauch, Institute for Systems Biology, 401 Terry Avenue North, Seattle, WA 98109, USA.Follow
Seamus R Morrone, Institute for Systems Biology, Seattle, WA. USA
Bradley P Yates
Alex U Singer
Jiefei Tong
Noah Manczyk
Gerald Gish
Zhi Sun, Institute for Systems Biology , Seattle , Washington 98008 , United StatesFollow
Igor Kurinov
Frank Sicheri
Michael F Moran
Robert L Moritz, Institute for Systems Biology, Seattle, Washington, United States of America.Follow
Sachdev S Sidhu

Document Type

Article

Publication Date

6-17-2022

Keywords

washington; isb; Humans; Mass Spectrometry; Phosphotyrosine; Protein Binding; Proteome; src Homology Domains

Abstract

A comprehensive analysis of the phosphoproteome is essential for understanding molecular mechanisms of human diseases. However, current tools used to enrich phosphotyrosine (pTyr) are limited in their applicability and scope. Here, we engineered new superbinder Src-Homology 2 (SH2) domains that enrich diverse sets of pTyr-peptides. We used phage display to select a Fes-SH2 domain variant (superFes; sFes

Specialty

Institute for Systems Biology

Recommended Citation

Martyn, Gregory D; Veggiani, Gianluca; Kusebauch, Ulrike; Morrone, Seamus R; Yates, Bradley P; Singer, Alex U; Tong, Jiefei; Manczyk, Noah; Gish, Gerald; Sun, Zhi; Kurinov, Igor; Sicheri, Frank; Moran, Michael F; Moritz, Robert L; and Sidhu, Sachdev S, "Engineered SH2 Domains for Targeted Phosphoproteomics." (2022). Articles, Abstracts, and Reports. 6360.
https://digitalcommons.providence.org/publications/6360