Analytic pipelines to assess the relationship between immune response and germline genetics in human tumors.
Publication Title
STAR Protoc
Document Type
Article
Publication Date
12-16-2022
Keywords
washington; isb; genomics; Humans; Genome-Wide Association Study; Neoplasms; Genome; Immunity; Germ Cells
Abstract
Germline genetic variants modulate human immune response. We present analytical pipelines for assessing the contribution of hosts' genetic background to the immune landscape of solid tumors using harmonized data from more than 9,000 patients in The Cancer Genome Atlas (TCGA). These include protocols for heritability, genome-wide association studies (GWAS), colocalization, and rare variant analyses. These workflows are developed around the structure of TCGA but can be adapted to explore other repositories or in the context of cancer immunotherapy. For complete details on the use and execution of this protocol, please refer to Sayaman et al. (2021).
Area of Special Interest
Cancer
Specialty/Research Institute
Institute for Systems Biology
Specialty/Research Institute
Oncology
