Characterization of murine lymphocyte activation and exhaustion markers by a 14-color flow cytometry panel.

Publication Title

Bioanalysis

Authors

Daniel Rose, Earle A. Chiles Research Institute, Robert W. Franz Cancer Center, Portland, OR, USAFollow
Annah S Rolig, Earle A. Chiles Research Institute, Providence Portland Medical Center, Portland, OR.Follow
William L. Redmond, Earle A Chiles Research InstituteFollow

Document Type

Article

Publication Date

5-1-2023

Keywords

oregon; chiles; T cell; assay; biomarkers; cytotoxic; exhaustion; flow cytometry; immunoassay; lymphocyte

Abstract

Previously designed flow cytometry panels have provided a framework to analyze T-cell activation; however, few provide an extensive view of lymphocyte populations, and none are optimized for murine models. This article describes a panel designed specifically to assess the expression of activation and exhaustion markers in expanding lymphocyte populations in tumor-bearing mice across two distinct genetic backgrounds

Clinical Institute

Cancer

DOI

10.4155/bio-2023-0005