Characterization of murine lymphocyte activation and exhaustion markers by a 14-color flow cytometry panel.

Authors

Daniel Rose, Earle A. Chiles Research Institute, Robert W. Franz Cancer Center, Portland, OR, USAFollow
Annah S Rolig, Earle A. Chiles Research Institute, Providence Portland Medical Center, Portland, OR.Follow
William L. Redmond, Earle A Chiles Research InstituteFollow

Document Type

Article

Publication Date

5-1-2023

Publication Title

Bioanalysis

Keywords

oregon; chiles; T cell; assay; biomarkers; cytotoxic; exhaustion; flow cytometry; immunoassay; lymphocyte

Abstract

Previously designed flow cytometry panels have provided a framework to analyze T-cell activation; however, few provide an extensive view of lymphocyte populations, and none are optimized for murine models. This article describes a panel designed specifically to assess the expression of activation and exhaustion markers in expanding lymphocyte populations in tumor-bearing mice across two distinct genetic backgrounds: BALB/c and C57BL/6. This comprehensive panel enables the assessment of multiple functional states and immune checkpoint markers across cytotoxic CD8

Clinical Institute

Cancer

Recommended Citation

Rose, Daniel; Rolig, Annah S; and Redmond, William L., "Characterization of murine lymphocyte activation and exhaustion markers by a 14-color flow cytometry panel." (2023). Articles, Abstracts, and Reports. 7318.
https://digitalcommons.providence.org/publications/7318