3-D chromatin conformation, accessibility, and gene expression profiling of triple-negative breast cancer.

Document Type

Article

Publication Date

11-2-2023

Publication Title

BMC Genom Data

Keywords

Humans; Triple Negative Breast Neoplasms; Chromatin; Cell Line, Tumor; Gene Expression Profiling; Breast; ATAC-seq; Chromatin accessibility; Epigenetic profiling; Hi-C; Long-range interactions; MDA-MB-231; MDA-MB-436; RNA levels; RNA-seq.; california; sjci; santa monica; genomics

Abstract

OBJECTIVES: Triple-negative breast cancer (TNBC) is a highly aggressive breast cancer subtype with limited treatment options. Unlike other breast cancer subtypes, the scarcity of specific therapies and greater frequencies of distant metastases contribute to its aggressiveness. We aimed to find epigenetic changes that aid in the understanding of the dissemination process of these cancers.

DATA DESCRIPTION: Using CRISPR/Cas9, our experimental approach led us to identify and disrupt an insulator element, IE8, whose activity seemed relevant for cell invasion. The experiments were performed in two well-established TNBC cellular models, the MDA-MB-231 and the MDA-MB-436. To gain insights into the underlying molecular mechanisms of TNBC invasion ability, we generated and characterized high-resolution chromatin interaction (Hi-C) and chromatin accessibility (ATAC-seq) maps in both cell models and complemented these datasets with gene expression profiling (RNA-seq) in MDA-MB-231, the cell line that showed more significant changes in chromatin accessibility. Altogether, our data provide a comprehensive resource for understanding the spatial organization of the genome in TNBC cells, which may contribute to accelerating the discovery of TNBC-specific alterations triggering advances for this devastating disease.

Clinical Institute

Women & Children

Clinical Institute

Cancer

Department

Oncology

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