Evaluation of solid-phase panreactivity with negative direct antiglobulin testing.

Document Type

Article

Publication Date

12-1-2023

Publication Title

Immunohematology

Keywords

Humans; Male; Female; Adult; Hemolysis; Coombs Test; Autoantibodies; Erythrocytes; Antibodies, Anti-Idiotypic; oregon; portland

Abstract

Solid-phase red cell adherence (SPRCA) is a sensitive platform for antibody detection, but nonspecific reactions may occur. One pattern of apparent nonspecific reactivity is a panagglutinin with a negative direct antiglobulin test (DAT). The purpose of this study was to define the clinical characteristics of patients with these nonspecific reactions and their associated serologic findings. Twenty patients with panreactive SPRCA testing results were identified between November 2022 and May 2023. In addition to panagglutinins, these patients had (1) a negative polyethylene glycol (PEG) antibody detection test, (2) a negative PEG autocontrol, and (3) a negative DAT. The strength of SPRCA panreactivity and the results of eluate testing (by tube and SPRCA) were studied. Clinical characteristics of patients included age, sex, and primary diagnosis. Each patient was also assessed for evidence of hemolysis. Fourteen female and six male patients were evaluated (average age 44 years). Primary diagnoses included pregnancy (

Department

Endocrinology

Department

Pathology & Laboratory Medicine

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