Isolation of Breast cancer CTCs with multitargeted buoyant immunomicrobubbles.

Publication Title

Colloids and surfaces. B, Biointerfaces

Authors

Guankui Wang
Halli Benasutti
Jessica F Jones
Guixin Shi
Michael Benchimol
Sandeep Pingle, Department of Translational Neuro-Oncology and Neurotherapeutics, John Wayne Cancer Institute at Providence Saint John's Health Center
Santosh Kesari, Department of Translational Neuro-Oncology and Neurotherapeutics, John Wayne Cancer Institute at Providence Saint John's Health CenterFollow
Yasan Yeh
Li-En Hsieh
Yu-Tsueng Liu
Anthony Elias
Dmitri Simberg

Document Type

Article

Publication Date

1-1-2018

Keywords

Antibody; Breast cancer; CTC; EGFR; EpCAM; Microbubbles; Biomarkers, Tumor/metabolism; Breast Neoplasms/blood; Breast Neoplasms/pathology; Cell Line, Tumor Cell Separation/methods; Epithelial Cell Adhesion Molecule/metabolism; Female; Humans; Keratins/metabolism; MCF-7 Cells; Microbubbles; Neoplastic Cells, Circulating/metabolism; Neoplastic Cells, Circulating/pathology

Abstract

Circulating tumor cells (CTCs) are extremely rare cells found in blood of metastatic cancer patients. There is a need for inexpensive technologies for fast enrichment of CTCs from large blood volumes. Previous data showed that antibody-conjugated lipid shell immuno-microbubbles (MBs) bind and isolate cells from biological fluids by flotation. Here, blood-stable MBs targeted to several surface markers for isolation of breast tumor cells were developed. MBs coated with anti-human EpCAM antibodies showed efficient binding of EpCAM

Area of Special Interest

Cancer

Specialty/Research Institute

Oncology