Integrated Antigenic and Nucleic Acid Detection in Single Virions and Extracellular Vesicles with Viral Content.
Publication Title
Adv Healthc Mater
Document Type
Article
Publication Date
5-31-2024
Keywords
washington; isb; swedish; swedish cancer; covid-19
Abstract
Virion-mediated outbreaks are imminent and despite rapid responses, continue to cause adverse symptoms and death. Therefore, tunable, sensitive, high-throughput assays are needed to help diagnose future virion-mediated outbreaks. Herein, it is developed a tunable in situ assay to selectively enrich virions and extracellular vesicles (EVs) and simultaneously detect antigens and nucleic acids at a single-particle resolution. The Biochip Antigen and RNA Assay (BARA) enhanced sensitivities compared to quantitative reverse-transcription polymerase chain reaction (qRT-PCR), enabling the detection of virions in asymptomatic patients, genetic mutations in single virions, and enabling the continued long-term expression of viral RNA in the EV-enriched subpopulation in the plasma of patients with post-acute sequelae of the coronavirus disease of 2019 (COVID-19). BARA revealed highly accurate diagnoses of COVID-19 by simultaneously detecting the spike glycoprotein and nucleocapsid-encoding RNA in saliva and nasopharyngeal swab samples. Altogether, the single-particle detection of antigens and viral RNA provides a tunable framework for the diagnosis, monitoring, and mutation screening of current and future outbreaks.
Clinical Institute
Cancer
Specialty
Oncology
Specialty
Infectious Diseases
Specialty
Institute for Systems Biology
Recommended Citation
Nguyen, Kim Truc; Rima, Xilal Y; Nguyen, Luong T H; Wang, Xinyu; Kwak, Kwang Joo; Yoon, Min Jin; Li, Hong; Chiang, Chi-Ling; Doon-Ralls, Jacob; Scherler, Kelsey; Fallen, Shannon; Godfrey, Stephanie L; Wallick, Julie A; Magaña, Setty M; Palmer, Andre F; Lee, Inyoul; Nunn, Christopher C; Reeves, Kimberly M; Kaplan, Henry G; Goldman, Jason D; Heath, James R; Wang, Kai; Pancholi, Preeti; Lee, L James; and Reátegui, Eduardo, "Integrated Antigenic and Nucleic Acid Detection in Single Virions and Extracellular Vesicles with Viral Content." (2024). Articles, Abstracts, and Reports. 8974.
https://digitalcommons.providence.org/publications/8974