Detection of micro metastasis of melanoma and breast cancer in paraffin-embedded tumor draining lymph nodes by multimarker quantitative RT-PCR

Authors

Dave S B Hoon, Department of Translational Molecular Medicine, John Wayne Cancer Institute (JWCI) at Providence Saint John's Health Center, Santa Monica, CA 90404 USA.Follow
Hiroya Takeuchi

Document Type

Patent

Publication Date

11-14-2003

Abstract

The invention provides a quantitative realtime RT-PCR assay for detection of metastatic breast, gastric, pancreas or colon cancer cells or metastatic melanoma. The assay allows to predict disease recurrence and survival in patients with AJCC stage I and II, and III disease using multimarker panels. The method for detecting metastatic melanoma cells utilizes panels of markers selected from a group consisting of MAGE-A3, GalNAcT, MART-1, PAX3, Mitf, TRP-2, and Tyrosinase. The method for detecting metastatic breast, gastric, pancreas or colon cancer cells in paraffin-embedded samples utilizes panels of markers selected from a group consisting of C-Met, MAGE-A3, Stanniocalcin-1, mammoglobin, HSP27, GalNAcT, CK20, and .beta.-HCG.

Area of Special Interest

Cancer

Specialty/Research Institute

Oncology

Location

United States

Comments

Patent Number: 7,910,295

Assignee

John Wayne Cancer Institute (Santa Monica, CA)

Family ID

32326322

Serial Number

10/713,808

Tech ID

02-JWCI-035