Detection of micro metastasis of melanoma and breast cancer in paraffin-embedded tumor draining lymph nodes by multimarker quantitative RT-PCR
Document Type
Patent
Publication Date
11-14-2003
Abstract
The invention provides a quantitative realtime RT-PCR assay for detection of metastatic breast, gastric, pancreas or colon cancer cells or metastatic melanoma. The assay allows to predict disease recurrence and survival in patients with AJCC stage I and II, and III disease using multimarker panels. The method for detecting metastatic melanoma cells utilizes panels of markers selected from a group consisting of MAGE-A3, GalNAcT, MART-1, PAX3, Mitf, TRP-2, and Tyrosinase. The method for detecting metastatic breast, gastric, pancreas or colon cancer cells in paraffin-embedded samples utilizes panels of markers selected from a group consisting of C-Met, MAGE-A3, Stanniocalcin-1, mammoglobin, HSP27, GalNAcT, CK20, and .beta.-HCG.
Area of Special Interest
Cancer
Specialty/Research Institute
Oncology
Location
United States
Assignee
John Wayne Cancer Institute (Santa Monica, CA)
Family ID
32326322
Serial Number
10/713,808
Tech ID
02-JWCI-035
Comments
Patent Number: 7,910,295